Bioindicators

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A Report on the Preference PH Of Isopods
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INTRODUCTION
Isopods (singular Isopoda) are a type of crustacean that are found on land or in the seas.
The terrestrial isopods are mostly found in cool moist places on land while the aquatic species
which are the majority are found in the bottom of freshwater bodies or on seabed’s. Terrestrial
isopods are commonly known as pill bugs or sow bugs. Similar to their aquatic relatives they
breath with gills. Despite their similarities they have different defense mechanisms, sow bugs
will normally try to flee while pill bugs roll themselves into a ball as a defense mechanism or for
conservation of moisture.
PH is the measure of the hydrogen ions concentration in an aqueous solution on a
numeric basis to specify the acidity or basicity of the solution. The use of pH. indicators has for a
long time helped in obtaining of information in various fields therefore the use of bioindicators
in our case is very helpful in learning and understanding the preferences of terrestrial isopods.
However, it is risky to rely on bioindicators entirely, just because a certain species is in acidic
soil it does not mean it is acidophilus or the reverse whereby an acidophilus species is found in a
certain soil it does not necessarily mean the soil is acidic. Laboratory experiments will therefore
be very helpful in minimizing the risk of wrong conclusions because the allow independent
assessment of the species.
In response to the environmental changes terrestrial isopods have shown different trends
that are related with their behavior, physiology and ecology. For example, for the isopods that
are from the arid or semi-arid regions, they are hydrokinetic at low humidifies and do not
respond well to photoreaction at lower temperatures (SASKA, PAVEL). They are also
negatively thermoactivated, meaning they are inactive in places of high temperatures. The
change in soil pH. brought about by use of fertilizers has shown to cause a great change in the
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behavior of isopods. The responses from terrestrial isopods are very instrumental and beneficial
in assessing any changes that might have taken place in the soil composition. exoskeletons of
these isopods that are still preserved can and are been used as a channel for the reconstruction of
the past soil ph. depending on species decomposition.
Bioindicators can tell us about the cumulative effects of pollutants in the ecosystem and
about how long they have been present which chemical and physical testing cannot which is one
major purpose. Hence an extensive number of experiments was carried out to test reproducibility
of preference distributions observed for different bioindicators. Another objective was to
measure the pH. of the soil and check some of the conditions that change pH., distinguish
between exchangeable and active acidity. To get a better understanding of the function of CO
2
in
determining the pH. of soil. Through carrying out of the various experiments the outcome should
be able to give us the different chemical stimulus that the isopods directly react to. An indicator
value was assigned to each different arthropod species to denote the specify of each response.
The preferred PH is expected to vary from a range of 2.5 to 8.0 since most species have a broad
preference distribution.
METHOD
For the experiment four different sponges. The length, width and heights of the sponges were
measured. The various solutions were prepared with acidities ranging from pH. 4.2 to 9.8
whereby the acidic dilution used was HCL 0.1M which was positive and the basic dilution used
was NAOH 0.1M which was negative. For the neutral dilution water was used. Constants such as
room temperatures were at 20 and the humidity of the air at 76% .Sponges were strictly placed
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in a downside manner in the arena {broadside flat} and separated by distance ranging from
different colours of the sponges which were green, yellow, purple and orange. The various
distance measures between each sponge were
green to orange: 10.0 cm
purple to yellow: 9.0 cm
yellow to green: 2.8 cm
green to purple: 2.9 cm
orange to purple: 3.6 cm
yellow to orange: 2.5 cm
The orange sponge represented a neutral solution, the purple sponge acidic, the yellow sponge
basic and the green sponge represented dry conditions. The distilled acidic, neutral and basic
solutions were poured on the various sponges dropwise using a dropper till the sponges were
soaked. There were changes in mass after this. An isopod was then placed in the same arena as
the sponges to see which environment the isopods would prefer most.
A timer was thereafter used to check the time elapsed for the different isopods to move to the
various sponges with different conditions. Trials conducted were four for the results to be
conclusive. Time taken for the isopods to reach the various sponges ranged from the shortest
period which was 4.10 to the longest period which was 49.22 seconds.
RESULTS
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The masses of the sponges definitely increased after the drops of liquid were dropped on them.
The table below clearly indicates the dimensions of the sponge, the mass recorded before and
after addition of liquid.
Length
(cm)
Width
(cm)
Height
(cm)
Volume
(cm^3)
Surface
Area
(cm^2)
Mass
Before
Liquid
(g)
Number
of drops
of liquid
1.95
0.18
4.50
1.58
1.5795
1.85
72
1.99
0.13
3.91
1.01
1.011517
1.36
0
1.96
0.10
4.38
0.86
0.85848
2.23
78
2.15
0.12
4.17
1.08
1.07586
1.98
68
For each isopod, four trials were done and the results are as seen below. The column heads
represent the isopod number and the two row heads represent the sponge preference of the
isopod and the time it took to choose that sponge respectively. A quarter of the 40 times the
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isopods chose the color purple. Orange was the least popular color among the isopods while
yellow and green was in close range of preference.
.
0
1
2
3
4
5
6
Green Orange Purple Yellow
Count of Isopod number by Sponge preference (color)
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Trial 1
Isopod number
Sponge preference (color)
Time for
choice (s)
1
Green
8.69
2
Purple
15.70
3
Purple
8.53
4
Purple
25.65
5
Yellow
38.38
6
Orange
15.56
7
Purple
4.10
8
Yellow
25.75
9
Purple
32.83
10
Green
41.01
Trial 2
Isopod number
Sponge preference (color)
Time for
choice (s)
1
Yellow
39.42
2
Orange
14.66
3
Orange
16.22
4
Purple
17.88
5
Purple
20.15
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6
Green
15.05
7
Yellow
15.03
8
Green
34.18
9
Orange
14.45
10
Green
25.65
Trial 3
Isopod number
Sponge preference (color)
Time for
choice (s)
1
Orange
7.33
2
Green
8.80
3
Orange
13.23
4
Purple
7.63
5
Purple
8.58
6
Purple
41.67
7
Orange
5.36
8
Yellow
49.22
9
Purple
23.37
10
Yellow
10.47
Trial 4
Isopod number
Sponge preference (color)
Time for
choice (s)
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DISSCUSION
The data collected in the experiment showed that purple was the most preferred color of the
isopods. The purple solution was prepared using the NaOH hence basic and this supports the
hypothesis that the isopods would prefer the basic environment. The graph below shows that the
isopods preferred the color green to the orange or yellow solution. The green solution provided a
neutral environment and the orange and yellow were of the solutions made of HCL.the isopods
use their antennas to sense the different solutions (Wagler, Ron). The unease of the isopods in
the acidic environment could be explained by the irritation that comes with the acidic solution
once it gets into their gills.
1
Purple
30.55
2
Purple
20.10
3
Yellow
13.54
4
Purple
20.44
5
Orange
9.30
6
Yellow
8.53
7
Yellow
10.40
8
Orange
12.73
9
Green
18.45
10
Purple
4.51
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References
SASKA, PAVEL. "Granivory In Terrestrial Isopods." Ecological Entomology, 2008, Wiley-
Blackwell, doi:10.1111/j.1365-2311.2008.01026.x.
Wagler, Ron. "Exploring Terrestrial Isopods: How Terrestrial Isopods' Behavior Can Influence
Survival And Reproduction." Science Scope, vol 040, no. 09, 2017, National Science
Teachers Association (NSTA), doi:10.2505/4/ss17_040_09_46.

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