When the G protein is unable to hydrolyze the GTP bounded alpha subunit, it results in
the over stimulation of the secondary messengers that aid in the downstream reactions.
By this way, it results in the over synthesis of a particular compound. Say, for example if
this G protein cannot hydrolyze itself to GDP, then, the Alpha subunit with the GTP will
activate the cyclic AMP at an exponential level and there my create n imbalance in the
glycogen metabolism (cyclic AMP is involved in Glycogen metabolism). Hence it results
in the over production of certain compounds and creates an imbalance in the
metabolism. Hence the downstream activates will be severely affected.
How will this affect the pathway in which the G protein functions? Would
activates both upstream and downstream of the G protein be affected?
When the GTP bounded alpha subunit cannot hydrolyze the GTP, the alpha subit
cannot be associated to its counterpart beta and gamma complex. By this way, the G
protein cannot associate itself and hence cannot be connected to the receptor thereby
reducing the upstream functions of the G protein. Moreover, the pathway with which the
G protein functions becomes a futile and the stimulated GTP containing Alpha subuit
will be providing its signal for the synthesis of other secondary factors. Hence this leads
to over stimulation of the reactions. When the GTP is hydrolysed to GDP, the alphs
subunit can bind to the beta and gamma complex and hence to the receptor there by
activating the G protein only when there is a stimulus. This whole cycle of activation and
deactivation of the G-protein is disturbed.
